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KMID : 0359719940120020317
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Journal of the Korean Neurological Association
1994 Volume.12 No. 2 p.317 ~ p.322
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Gene Analysis of Probable Huntington's disease
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김만호
이광우/박경석/전범석/이상복/명호진/홍민아/서유보
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Abstract
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Increased CAG repeats with fullgene sequence (IT15) in Huntington's disease (HD) became known in 1993. We used polymerase chain reaction (PCR) in patients with clinically diagncsed Huntington's disease.
After extraction of genomic DNA from patient's blood, we amplified mutation site containing CAG repeats by PCR method using double 30mer primers. Agarose gel eletrophoresis with DNA ladder marker was performed and fluorescent staining was done
using
EtBr solution. Clinically diagnosed HD patients showed band at the same level that was not different from that of normal control. This suggests that these patients do not have increased CAG repeats in amplified site and means that they are
probably
not
Huntingto's disease. by PCR method with primers in flanking area.
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KEYWORD
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